Composite

Part:BBa_K5323894:Design

Designed by: Yidan Hu   Group: iGEM24_CUG-China   (2024-10-01)


Ptac-RBS-golS-Pgol-RBS-gfp-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1635
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3721
    Illegal AgeI site found at 1969
    Illegal AgeI site found at 2318
    Illegal AgeI site found at 3256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4570


Design Notes

This component is used to test the specificity and sensitivity of gold binding. And with Acidithiobacillus ferrooxidans as the chassis cell, we carried out codon optimization.


Source

A composite part assembled by Ptac (BBa_K180000),RBS(BBa_J34801), golS (BBa_K4468005), Pgol(BBa_K4468001), gfp(BBa_E0040) and TT(BBa_B0015).


References

Wei, W., Zhu, T., Wang, Y., Yang, H., Hao, Z., Chen, P. R., & Zhao, J. (2012). Engineering a gold-specific regulon for cell-based visual detection and recovery of gold. Chemical Science, 3(6), 1780-1784. Ruiz L M, Castro M, Barriga A, et al. The extremophile Acidithiobacillus ferrooxidans possesses ac‐di‐GMP signalling pathway that could play a significant role during bioleaching of minerals[J]. Letters in applied microbiology, 2012, 54(2): 133-139.